layiq

/la·yiq/adjective

worthy; deserving; fitting; suitable.

A role, opportunity, or path that merits attention, time, and pursuit.

LAYIQ - Gene Editing Lab Technician (2nd Shift) at Ohalo

Protoplast Isolation, Transfection, and Culture: Perform enzymatic digestion of plant tissues (e.g., leaf, root, or callus) to isolate viable protoplasts.
Transfect protoplasts using PEG-mediated transfection or electroporation methods.
Culture transfected protoplasts to regenerate callus
Contribute to the continuous improvement and documentation of SOPs for protoplast workflows.
Microscopy and Imaging: Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy and viability dyes (e.g., FDA staining).
Data Recording and Analysis: Maintain accurate and detailed electronic lab notebooks (ELN) and batch records.
Compile and analyze experimental data using tools such as ImageJ, GraphPad Prism, or Excel.
Sample Preparation: Prepare samples for downstream molecular analyses, including RNA extraction, protein extraction, and genomic DNA isolation.
Cross-Functional Support and Collaboration: Collaborate with other Technicians, Research Associates, and Scientists on workflows, ensuring that critical processes are completed.
Lab Maintenance and Compliance: Follow safety protocols and institutional guidelines for working with plant tissue and enzymes.
Perform all tasks in a manner consistent with and in support of all company policies and practices related to safety and compliance.
Performs other duties as assigned
Candidate Profile: Ability to accurately follow verbal and written instructions as well as make suggestions to optimize methodologies
Ability to sit for extended periods of time and do repetitive motions
Ability to take a proactive approach to routine tasks
Must be passionate about learning and enthusiastic about plants and hands-on work
Must have a positive attitude and good interpersonal skills
Ability to ask clarifying questions
Ability to work independently and in a team environment with multiple departments
Must be able to lift up to 50 pounds on intermittent basis and possess the ability to sit for extended periods of time
Candidate must prioritize safety
Desired skills/experience: Experience working in a lab including sterile technique, micropropagation, media preparation, microscopy, and DNA extraction

Gene Editing Lab Technician (2nd Shift)

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