layiq

/la·yiq/adjective

worthy; deserving; fitting; suitable.

A role, opportunity, or path that merits attention, time, and pursuit.

LAYIQ - Cell Assay Senior Scientist - Blue Sky Kinases at St. Jude

Research kinase-mediated signaling pathways, kinase targets or kinase-dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects
Identify appropriate cellular assays and cells to perform structure-guided analysis of kinase signaling output and cellular phenotypes related to structure-guided perturbations in kinase regulation or function
Develop strategies and plans for implementing cell-based assays, including but not limited to evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT-PCR), protein ubiquitination, and protein abundance
Develop strategies and plans for implementing cell-based phenotypic assays, including but not limited to viability, apoptosis, autophagy, migration, or differentiation
Perform optimization and troubleshooting of cell-based assays
Perform statistical analysis using graphing software or R scripts
Interpret, analyze, and communicate cell-based assay results within the Cell-Based Assays team and with collaborators on specific kinase projects
Experience and Expertise: Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways.
Expertise in culturing a variety of types of cells, such as cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells.
Expertise in multiple methods for exogenous protein expression.
Proficiency in presentation of experimental design and results using PowerPoint or similar software.
Experience with mouse models, preferred but not essential.
Technical Capabilities: Cloning, PCR, and qRT-PCR
Sterile technique
Cell transfection, transduction, or infection to exogenously express proteins of interest transiently or for cell line generation
Quantitative immunoassays, such as ELISA, AlphaLISA, and Mesoscale Discovery
Immunoprecipitation and Western blotting
Protein-protein interaction analysis, such as proximity ligation, FRET, or NanoBit assays
Flow cytometry and fluorescence-activated cell sorting (FACS)
Reporter gene assays
Phenotypic assays, such as viability, apoptosis, proliferation, migration, colony-forming, autophagy, endocytosis, secretion, or differentiation assays
Microscopy, such as immunofluorescence, live-cell imaging, subcellular localization, colocalization analysis
Cellular fractionation
Competencies: Creative and results-driven with strong critical thinking and the ability to learn quickly
Hardworking, well-organized, flexible, and capable of multitasking efficiently in a fast-paced environment
Collaborative and efficient member of teams with diverse backgrounds and opinions
Ability to prioritize work and complete assignments promptly
Minimum Education and Experience: Bachelor's degree in relevant scientific area is required.
Minimum Experience: Five (5) years of relevant work experience is required with a PhD.
Eight (8) years of relevant experience is required with a Master's Degree.
Ten (10) years of relevant work experience is required with a Bachelor's Degree.
The World’s Brightest Minds Always Innovate
At St. Jude Children’s Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That’s why we provide
World-class facilities
State-of-the-art technologies
Extraordinary support
A collaborative, bench-to-bedside environment
You will see, firsthand, how your science translates into survival for the children that we serve. Quite simply, at St. Jude, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children.
Better at Work. Better at Life.
When you work at St. Jude, you’ll join a highly collaborative work culture that inspires you every day to be your best.  With opportunities for learning and growth, you can shape a career path that is right for you while also enjoying all the benefits and stability of working for a world-class institution. This includes work-life balance with generous paid time-off and on-campus conveniences that make life a little easier. Join us and you’ll quickly see why St. Jude is consistently ranked by our employees on Glassdoor as a “Best Place to Work.”
Have More in Memphis
Get the best of big-city amenities mixed with all the charm of a small-town feel when you live in Memphis. From our world-renowned music scene to our eclectic mix of food and people, Memphis is a place our employees are proud to call home. Our region enjoys a cost of living more than 20% lower than the national average and our state places no personal income tax on wages. Plus, Memphians spend five hours less per year in their daily commute compared to the national average. Learn how you can “ Have More in Memphis .”

Cell Assay Senior Scientist - Blue Sky Kinases

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